Hyperspectral imaging/reflectance spectroscopy of mouse oral tissue exposed to high intensity blue light.
Oral tissues are exposed to optical radiation during various dental treatments and diagnostic methods. However, adverse effects are seldom addressed. Hyperspectral imaging (HSI) and visible near-infrared reflection spectroscopy (VNIRS) were used to observe oral tissue changes in mice exposed to blue light with respect to erythema (erythema index; EI) and pigmentation (melanin index; MI). Pigmented mice (C57BL/6N; ngroup = 3) were exposed on the tongue and the abdominal skin for control or left unexposed. Irradiation was performed with an LED intended for photopolymerisation of dental materials (λpeaks: 409/460 nm) with irradiance ≈ 2 W/cm2 and radiant exposure ≈ 120 J/cm2.