Microbiology testing and research -

The Colony-Forming Unit (CFU) method can be used for identification of microorganisms and for testing the effect of antimicrobial substances and techniques. It is a sensitive and widely accepted method within microbiology. In this method, the number of living cells (cells able to reproduce) present in a suspension is counted on a nutrient agar surface.

HET-CAM test -

HET-CAM test (hen’s egg-chorioallantoic membrane test) is a test used to determine the irritation potential of substances and is an alternative test to the Draize Rabbit Eye Test. In the test, incubated hen’s eggs are opened carefully on day 9 and the chorioallantoic membrane (CAM) is exposed.

Antibacterial effects of native and methacrylate modified chitosan -

Commercial resin-based composites are mainly composed of dimethacrylate-based monomers, such as triethyleneglycol dimethacrylate (TEGDMA), inorganic fillers and a coupling agent. Cured resinbased composites have no antibacterial effect against oral bacteria and half of all fillings replaced are because of new caries . Chitosan is a natural carbohydrate polymer derived from the deacetylation of chitin. Chitosan has been shown to have an inhibitor effect on the adherence of oral bacteria onto human tooth surfaces.

Role of thiol-complex formation in 2-hydroxyethyl-methacrylate-induced toxicity in vitro -

Methacrylate monomers that are found to leach from cured resin-based dental materials induce biological effects in vitro. The underlying mechanisms have not been fully elucidated although involvement of increased cellular reactive oxygen species (ROS) and DNA-damage has been suggested