In vitro element release and biological aspects of base-metal alloys for metal-ceramic applications -

Abstract Objective The aims of this study were to investigate the release of element from, and the biological response in vitro to, cobalt–chromium alloys and other base–metal alloys used for the fabrication of metal-ceramic restorations. Material and methods: Eighteen different alloys were investigated. Nine cobalt–chromium alloys, three nickel–chromium alloys, two cobalt–chromium–iron alloys, one palladium–silver alloy, […]

Cell toxicity of 2-hydroxyethyl methacrylate (HEMA): the role of oxidative stress -

In this study, the viability of human lung epithelial cells, BEAS-2B, was investigated after exposure to this monomer. Exposure to HEMA reduced the viability of the BEAS-2B cells as a result of increased apoptosis, interruption of the cell cycle, and decreased cell proliferation.

New method: Skin irritation test for evaluation of medical device extracts -

Although questions have been raised regarding reproducibility and animal welfare, the rabbit Draize eye test has remained an accepted method to evaluate skin irritation for many years. For the last decade, in vitro 3D skin models are developed to predict and classify skin irritation potential of chemicals. Several methods have been validated by ECVAM (European Centre for the Validation of Alternative Methods) and achieved regulatory acceptance internationally for replacement of the rabbit Draize test. Among the approved skin systems is the Epiderm™ system from Matek.

Cell toxicity of methacrylate monomers-the role of glutathione adduct formation. -

Polymer-based dental restorative materials are designed to polymerize in situ. However, the conversion of methacrylate monomer to polymer is never complete, and leakage of the monomer occurs. It has been shown that these monomers are toxic in vitro; hence concerns regarding exposure of patients and dental personnel have been raised.

Flow cytometry -

Flow cytometry is a technology that simultaneously measures and then analyzes multiple characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light.