Biotransformation enzymes and lung cell response to 2-hydroxyethyl-methacrylate.
Jan Tore Samuelsen, Jørn A. Holme, Marit Låg, Per E. Schwarze, Jon Einar Dahl and Rune Becher | November 29th 2011
The aim of this in vitro study was to investigate possible involvement of cytochrome P450 (CYP) enzymes in modifying the toxic potential of 2-hydroxyethyl-methacrylate (HEMA). Primary cultures of CYP expressing rat alveolar type 2 cells were exposed to varying concentrations of HEMA. Nuclear translocation of aryl hydrocarbon receptor (AhR) after HEMA exposure (100 μM) was demonstrated by immunocytochemical staining.