A laboratory investigation indicated that the cell-damaging potential seemed to match the degree of leaching of particular polymer-based endodontic materials. The methacrylate-based polymeric root canal sealing materials were responsible for this effect: Even the cured materials leached components and caused cells of oral origin to die when kept in contact with material extracts. The severity of cell death increased with the time that the material was available in the cell culture.
Evaluation of the biocompatibility of dental materials may be a complex task. Chemical analysis using in vitro investigations may be helpful to elucidate the biocompatibility of the materials through investigation and control of eluted substances. The aim of such investigations is to increase patient safety.